GLaDOS

GLaDOS (Genetic Lifeform and Disk Operating System) is the central core designed to control, guide, and oversee the Aperture Science computer-aided Enrichment Center. With the ability to shift and move facilities and various chambers, GLaDOS is able to achieve almost infinite testing design meanwhile residing in the Central AI Chamber.

In possession of a feminine programming, she is the antagonist of Portal and the first half of the single-player campaign in Portal 2. In the game's cooperative campaign, GLaDOS is the testing supervisor of Atlas and P-body.